Alberobello and Locorotondo are perfect for bike tours because they offer scenic countryside, charming historic centers, and unique attractions like the iconic trulli houses. Riding through these towns lets you explore places that are often inaccessible by car, such as narrow alleyways and unpaved country paths. We’ve picked some fantastic options, like the Electric Bike Villages of Apulia tour, which combines comfort and culture, and the e-Bike tours with wine tastings that let you sip local wines among vineyards. Whether you’re after a relaxed ride, a culinary experience, or a deeper dive into historic sites, there’s something here that fits your style.
Below, you’ll find a curated list of 15 top bike tours in the region—each with its own twist, from private guided rides to self-guided GPS adventures. Read on to find your perfect way to see Valle d’Itria’s rolling hills, trulli villages, and delicious local flavors.
1. Excursion Electric Bike Villages of Apulia

At number 1, the Excursion Electric Bike Villages of Apulia offers a 3.5-hour private ride through Alberobello, Locorotondo, and Martina Franca. The tour includes E-Bike and helmet hire, making it ideal if you want to travel farther with less effort. You’ll pedal along hidden, non-traffic roads that reveal the area’s charming trulli houses and scenic vineyards. The highlight is the visit to Alberobello’s famous Rione Monte and the church of Sant’Antonio, along with free time in Locorotondo’s picturesque center.
What makes it special: The private setting allows a personalized experience, and reviews praise the smooth bikes and engaging guide Martino, who shares interesting tidbits about local history. Many find the ride “magical” and appreciate the mix of scenic countryside and cultural highlights. The 30-minute coffee break in Alberobello is just enough to soak in the atmosphere without feeling rushed.
Bottom Line: Perfect for travelers seeking a comfortable, guided introduction to Valle d’Itria’s most iconic villages with flexible, private attention.
2. Ebike Tours: the Villages of Valle d’Itria and Tasting of Typical Products

At number 2, this 4-hour tour balances scenic riding with culinary delights. Starting from Martina Franca, you’ll explore Cisternino’s historic center, then cycle along the Apulian Aqueduct Cycle Route, famous for its 500 km of waterworks and beautiful countryside. The tour includes a tasting of local products at Masseria Madonna dell’Arco—think fresh, farm-to-table goodies like cheese and preserves.
What makes it special: The highlight is the blend of nature, history, and taste—cycling through peaceful rural roads, crossing the aqueduct, and ending with a farm-fresh meal. The guide Damiano ensures a safe, enjoyable ride, and many reviews mention the high quality of the bikes and the excellent local food.
Who it’s for: Food lovers who want to combine exercise with authentic local flavors, plus those interested in historic waterworks.
Bottom Line: Great value for those who want a well-rounded experience of Valle d’Itria’s scenery and cuisine in a single trip.
3. Bike Oil Tour in Monopoli

Number 3 focuses on the olive oil heritage of Monopoli, with a 4- to 5-hour mountain biking adventure starting at the Portavecchia promenade. You’ll visit centuries-old olive groves, rock churches, and an underground oil mill—an immersive look into the region’s production methods. The ride includes tasting local olive oils and Apulian products at the end.
What makes it special: Cycling through centuries-old olive trees and visiting hidden farms sets this apart. The tour’s reviews highlight the guide Antonella’s passion and the opportunity to see off-the-beaten-path places that reveal the area’s authentic flavor.
Who it’s for: Olive oil enthusiasts and those eager to combine a scenic ride with a taste of local craftsmanship.
Bottom Line: This is a tasty, authentic experience for anyone interested in regional food production and historic farms.
4. E-Bike, Focaccia and Wine

Number 4 delivers a 3-hour journey through country roads among trulli and farmhouses. Starting in Alberobello, you pedal 20 km to Locorotondo, where a focaccia tasting at a local bakery and a glass of wine await. The ride includes visits to key sites like Alberobello’s church of Sant’Antonio and a stroll through Locorotondo’s charming streets.
What makes it special: The combination of cycling, local food, and wine makes this tour stand out. Many reviews praise the tastings and the relaxed pace, making it ideal for those who want to combine gentle exercise with authentic flavors.
Who it’s for: Food and wine lovers who prefer a shorter, laid-back ride with delicious stops.
Bottom Line: A delightful way to experience Puglia’s culinary delights while enjoying scenic country landscapes.
5. Ebike Tour Alberobello and Villages Puglia

Ranked 5th, this 3.25-hour private tour starts from Martina Franca and includes stops at the Trulli of Alberobello and Locorotondo. The focus is on learning about the history and culture, with bike rental and helmet included. The itinerary emphasizes less traveled paths and photo opportunities of hidden conical roofs.
What makes it special: Its private customization and the chance to explore less crowded areas make it perfect for travelers who want a personalized experience. The reviews mention the friendly guide and the scenic, traffic-free paths.
Who it’s for: Those wanting an intimate, flexible ride that delves into local culture and photogenic spots.
Bottom Line: Excellent for travelers seeking a tailored, scenic exploration of Alberobello and Locorotondo.
6. GPS Self-Guided Alberobello and Masseria + Mozzarella and Focaccia Tastings

In 6th place, this GPS-guided experience combines self-exploring with culinary tastings. Starting from Locorotondo, you navigate Alberobello’s UNESCO-listed streets, enjoying famous trulli, then continue to Selva di Fasano for panoramic views and a Mozzarella and focaccia tasting.
What makes it special: The self-guided GPS means you set your own pace while enjoying authentic sights and tastes. The reviews praise the spectacular views and easy navigation.
Who it’s for: Confident explorers who prefer flexibility and want to focus on photo opportunities and local flavors.
Bottom Line: A great choice for independent travelers wanting a personalized, scenic, and flavorful day.
7. Ebike Tours: Alberobello and the Wines of Valle d’Itria

Number 7 offers a 4-hour guided ride through the vineyards and olive groves of Valle d’Itria. The highlight is visiting an 18th-century winery for a wine tasting after exploring Alberobello’s trulli neighborhoods.
What makes it special: This tour combines active cycling with wine culture, giving you access to hidden wineries that are often missed on other trips. Many reviews mention the “fabulous” winery experience and scenic vineyards.
Who it’s for: Wine lovers who want a gentle bike ride paired with tastings at historic estates.
Bottom Line: Ideal for those wanting to combine cycling and wine in a relaxed and informative way.
8. e-Bike Tour from Monopoli to the Rocky Village of S. Andrea

Ranked 8th, this 3.5-hour e-bike ride along the coast explores Monopoli’s old town, caves, and rocky village of S. Andrea. It includes stops at ancient rock churches and beach coves—perfect for those who enjoy coastal scenery.
What makes it special: The combination of historic sites and seaside views creates a varied experience. Reviewers highlight the knowledgeable guide Rocco and the spectacular cave village.
Who it’s for: Those who love coastal landscapes and cultural sites in a manageable ride.
Bottom Line: A balanced ride that blends history, geology, and seaside scenery.
9. e-bike Tour along the Cycle Path of the Apulian Aqueduct

Number 9 is a 3-hour self-guided cycle exploring the longest aqueduct in Europe. It passes through vineyards, historic towns, and scenic viewpoints, ending with a local wine tasting in Locorotondo.
What makes it special: Cycling along the historic aqueduct combines engineering marvels with lovely scenery. It’s praised for easy navigation and the quality bikes, according to reviews.
Who it’s for: Those interested in historic infrastructure combined with local wine and scenic countryside.
Bottom Line: Perfect for tech-savvy travelers wanting a self-guided, scenic, and cultural experience.
10. Smart & Easy Ebike Tour in the Itria Valley: Martina Franca – Locorotondo

Number 10 is a short, 1 to 1.5-hour tour designed for those with limited time. You’ll ride from Martina Franca to Locorotondo, enjoying picturesque streets and views of the valley with a friendly guide.
What makes it special: Its speed and simplicity make it ideal for travelers who want a quick but memorable experience.
Who it’s for: Visitors on a tight schedule who still want a taste of Valle d’Itria.
Bottom Line: Best suited for casual riders wanting an easy introduction to the area.
11. Monopoli’s Countryside by Bike

Ranked 11, this 4-hour guided tour visits Monopoli’s old town, olive groves, and medieval churches like Spirito Santo. The ride ends with artisanal ice cream tasting.
What makes it special: It offers a combination of cultural sites and scenic farmland, with a knowledgeable guide Rocco and a focus on local history and food.
Who it’s for: Travelers wanting to explore both urban and rural environments in one trip.
Bottom Line: A well-rounded tour for those interested in history, countryside, and food.
12. GPS Tour with E-Bike on Cycleroute of the Aqueduct with Wine Tasting

Number 12 offers a 3-4 hour self-guided ride along the famous aqueduct. It features breathtaking views, photo stops, and concludes with a wine tasting in Locorotondo.
What makes it special: The self-guided GPS system makes it flexible to explore at your own pace, with excellent reviews praising bike quality and scenic routes.
Who it’s for: Independent travelers seeking scenic views and local wine in a relaxed setting.
Bottom Line: Great for adventurers who prefer to navigate their own adventure.
13. E-bike Ring Tour between Locorotondo and Martina Franca

Ranked 13, this 3-hour round trip takes you through Locorotondo’s historic streets, countryside vistas, and Martina Franca’s charming old town. It ends with homemade ice cream.
What makes it special: The loop format covers both towns and breathtaking landscapes, with a chance to enjoy local ice cream at the end—an excellent finish.
Who it’s for: Travelers who want a balanced circular route with cultural and scenic highlights.
Bottom Line: Ideal for those wanting a comprehensive, relaxed ride with a sweet ending.
14. e-Bike Tour in Locorotondo, Oil Mill Visit, and EVO Oil Tasting

Number 14 includes a 3-hour scenic ride through Locorotondo’s streets, ending with a visit to a historic oil mill and a tasting of EVO olive oil.
What makes it special: The combination of cycling and learning about olive oil production makes this tour both educational and delicious. Visitors love the guided visit and the authentic oil tasting.
Who it’s for: Foodies and those eager to understand regional olive oil craftsmanship.
Bottom Line: A distinctive, flavorful experience blending culture, cuisine, and scenery.
15. Ebike: Authentic Itria Valley and Local Cuisine

Last on our list, this 4-hour guided ride explores Valle d’Itria’s scenic landscapes, ending with local dishes like orecchiette and mozzarella paired with local wine. The tour visits tratturi and ends in a stunning district with tastings.
What makes it special: It’s a culinary-focused tour with local food stops and authentic scenery. Reviewers mention the delicious food and beautiful landscapes.
Who it’s for: Food lovers wanting a full sensory experience of Puglia’s flavors and scenery.
Bottom Line: Perfect for those seeking an immersive taste of local cuisine combined with riding scenic roads.
How to Choose Your Perfect Bike Tour
When selecting a bike tour in Alberobello or Locorotondo, consider your fitness level, interest in food or wine, and how much time you want to spend cycling. For a relaxed introduction, the Smart & Easy Ebike Tour (link) is ideal. If your passion is culinary and wine tasting, look at the Ebike Tours with wine tasting (link) or olive oil experiences (link). For those craving self-guided adventures, the GPS tours (link) offer maximum flexibility.
Budget-conscious travelers should note that most tours range from about $43.94 to $122.13, often including bike rental and tastings. Book early in the season, especially in spring and early fall when the weather is most agreeable for biking.
Final Thoughts
Whether you’re after a scenic ride through historic villages, a culinary journey with local wines and olive oils, or an active day exploring the countryside, Alberobello and Locorotondo have a bike tour for you. For a relaxed, intimate experience, the private Excursion Electric Bike Villages of Apulia (link) is hard to beat. For those wanting to combine cycling with food and wine, the Valle d’Itria wine tour (link) offers a memorable pairing.
No matter your choice, these tours promise an immersive way to see the region’s unique landscapes and authentic flavors. Reserve your spot early—these experiences fill quickly, especially in peak season.